Staphylococcus epidermis often infects hospital patients with weak immune systems in catheter wounds European Bioinformatics Institute.
Escherichia coli, Bacillus cereus, Proteus vulgaris, Staphylococcus epidermis, Enterobacter aerogenes, and Pseudomonas fluorescens. As shown, it was Figure 8 is Bacillus cereus for glucose. They are shaken together vigorously and set aside for about one hour until the results can be read.
The Bacillus cereus exhibited a lot of growth and turned the milk agar clear, so it was protease positive Figure The Proteus vulgaris exhibited growth, so it is Gram negative, and it does not ferment lactose Figure TOP Urease test This test is used to identify bacteria capable of hydrolyzing urea using the enzyme urease.
The iodine reacts with the starch to form a dark brown color. It is used to determine if an organism is capable of reducing nitrate NO3- to nitrite NO2- or other nitrogenous compounds via the action of the enzyme nitratase also called nitrate reductase.
The second selective ingredient is sodium azide. Finally, failing to inoculate the SIM tubes.
This is considered a positive result. In addition, the outer membrane contains a lipid bilayer, lipoproteins, polysaccharides, and lipopolysaccharides. Specific chemicals located on the tips of pili enable organisms to attach to selected host tissue sites eg, urinary tract endothelium. Generally, if the entire tube is turbid, this indicates that the bacteria have moved away from the stab mark are motile.
Escherichia coli is capable of fermenting glucose as are Proteus mirabilis far right and Shigella dysenteriae far left. Proteus mirabilis pictured here, second from right is a glucose positive, lactose negative, sulfur reducing enteric.
The organism pictured on the far left is positive for hydrogen sulfide production. The Enterobacter aerogenes had a slightly acidic pH and no carbon dioxide gas was given off Figures 3, 5, and Then, using aseptic technique, inoculate each bacteria into each color tube by sticking the inoculating loop to the bottom of the tube and twirling it, then pulling it straight out.
The hydrolysis of urea forms the weak base, ammonia, as one of its products. SIM tubes are inoculated with a single stab to the bottom of the tube.
As shown, the Pseudomonas fluroescens turned purple. In the oxidase test, artificial electron donors and acceptors are provided. Bacillus subtilis is positive for starch hydrolysis pictured below on the left. Microbiology 20 Biochemical Unknown – Spring (due May 14th) You should be prepared to turn in your notebook with your biochemical unknown identification completed after lab on Thursday May elleandrblog.com of a possible 70 points you.
Microbiology 20 Biochemical Unknown – Spring (due May 14th) You should be prepared to turn in your notebook with your biochemical unknown identification completed after lab on Thursday May elleandrblog.com of a possible 70 points you.
It was concluded that P. vulgaris was the unknown. To confirm, the To confirm, the bacterium was plated on a desoxycholate plate as a differential test for P. vulgaris. The only TWO POSITIVES for Urease are Proteus vulgaris and Staph epidermidis, all the rest are negative.
Nitrate Test Results ALL organisms are Nitratase POSITIVE, However, Pseudomonas aeruginosa and Staohylococcus epidermidis are Nitritase NEGATIVE while all the REST are POSITIVE.
"Proteus Vulgaris Unknown Lab Report" Essays and Research Papers Proteus Vulgaris Unknown Lab Report Unknown Lab Report Dr. Nathan Cahoone Microbiology December 9, Introduction There are many reasons for knowing the identity of microorganisms.
Proteus vulgaris is a rod-shaped, nitrate-reducing, indole+ and catalase-positive, hydrogen sulfide-producing, Gram-negative bacterium that inhabits the intestinal tracts of humans and animals.
It can be found in soil, water, and fecal matter.Unknown is proteus vulgaris